Fig. 4
From: SIRT2 and ALDH1A1 as critical enzymes for astrocytic GABA production in Alzheimer’s disease
SIRT2 is essential, while ALDH1A1 is partially involved in GABA production. A Schematic for working of 2-cell sniffer patch experiments (Left) Representative fluorescence images of the sniffer patch experiment (Right top, scale bar 40 μm) and representative trace of full activation of GABAC-expressing HEK cells by GABA treatment (Right bottom). B Experimental timeline for 2-cell sniffer patch experiments with pharmacological inhibition (Left) or genetic ablation (Right) of target enzymes. C Representative traces (Left) and bar graph (Right) of GABA release-mediated sensor current from astrocytes treated with or without putrescine in the presence or absence of DEAB or AGK2. D Representative traces (Left) and bar graph (Right) of GABA release-mediated sensor current from putrescine-treated cultured astrocytes expressing Scr/shALDH1A1/shSIRT2-mCherry. Data represents Mean ± SEM. Individual data points represent each cell recorded. Values above graphs represent p-value from corresponding statistical test (Ordinary One-way ANOVA)